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Coordinated and published by The Grassroots Institute, the Grassroots Journal of Natural Resources (GJNR) is an international journal dedicated to the latest advancements in natural resources throughout the world. The goal of this journal is to provide a platform for scientists, social scientists, policy analysts, managers and practitioners (on all academic and professional levels) all over the world to promote, discuss and share various new issues and developments in different arenas of natural resources.
Ghassan Zaid Mureef*1, Ammar Waleed Taha2, Khaled Abdullah Sahar3, Ali Ahmed Ali4
1Department of Horticulture and Landscape, College of Agriculture, University of Samarra, Iraq.
Email: ghasan.zaid.m@uosamarra.edu.iq | ORCID: https://orcid.org/0009-0004-2631-9527
2Department of Horticulture and Landscape, College of Agriculture, University of Tikrit, Iraq.
Email: ammar.w.taha@tu.edu.iq | ORCID: https://orcid.org/0009-0009-4993-9530
3Department of Horticulture and Landscape, College of Agriculture, University of Samarra, Iraq.
Email: khalid.a.s@uosamarra.edu.iq | ORCID: https://orcid.org/0000-0002-7991-1143
4Department of Horticulture and Landscape, College of Agriculture, University of Samarra, Iraq.
Email: ali.a@uosamarra.edu.iq | ORCID: https://orcid.org/0009-0007-5196-1070
*Corresponding author
Grassroots Journal of Natural Resources, 9(1): 126-140. Doi: https://doi.org/10.33002/nr2581.6853.090106
Received: 23 October 2025
Reviewed: 22 December 2025
Provisionally Accepted: 15 November 2025
Revised: 19 January 2026
Finally Accepted: 23 February 2026
Published: 29 April 2026
The experiment was carried out in the wooden greenhouse at the Faculty of Agriculture / Samarra University, for the growing season from July 2022 to June 2023. A total of 108 seedlings (1.5 years old) of grapefruit and shaddock seedlings, grafted onto sour orange rootstock, to evaluate the effect of the leaf and nutrient solution on anatomical characteristics. The treatment included three concentrations of JA (0, 4, and 8 mmol L-1) and three levels of nutrient solution (0, 2.5, and 5 ml L-1). Spraying was carried out in three rounds, with the first application followed by two subsequent treatments at 21‑day intervals. This study was carried out according to the design of the Randomized Complete Block Design (RCBD) with three factors: two citrus types (grapefruit and shaddock), a Jasmonates (JA) regulator, and a nutrient solution, with three seedling replicates per treatment. The results were analyzed using ANOVA with SAS software, and the least significant difference (LSD) at a probability level of 0.05 was used for pairwise comparisons. The grapefruit showed a significant increase in stomatal density at (182.8 mm-2), while shaddock recorded higher values in cell dimensions.
Jasmonic acid; Nutrient solution; Grapefruit; Shaddock; Anatomical traits; Stomata
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Mureef, G.Z., Taha, A.W., Sahar, K.A. and Ali, A.A. (2026). The Effect of Spraying with Jasmonic Growth Regulator and Nutrient Solution on the Anatomical Characteristics of Grapefruit and Shaddock Seedlings and Their Interference. Grassroots Journal of Natural Resources, 9(1): 126-140. Doi: https://doi.org/10.33002/nr2581.6853.090106
Mureef, G.Z., Taha, A.W., Sahar, K.A., & Ali, A.A. (2026). The Effect of Spraying with Jasmonic Growth Regulator and Nutrient Solution on the Anatomical Characteristics of Grapefruit and Shaddock Seedlings and Their Interference. Grassroots Journal of Natural Resources, 9(1), 126-140. https://doi.org/10.33002/nr2581.6853.090106
Mureef G.Z., Taha A.W., Sahar K.A., Ali A.A. The Effect of Spraying with Jasmonic Growth Regulator and Nutrient Solution on the Anatomical Characteristics of Grapefruit and Shaddock Seedlings and Their Interference. Grassroots Journal of Natural Resources, 2026, 9 (1), 126-140. https://doi.org/10.33002/nr2581.6853.090106
Mureef, Ghassan Zaid, Taha, Ammar Waleed, Sahar, Khaled Abdullah, Ali, Ali Ahmed. 2026. “The Effect of Spraying with Jasmonic Growth Regulator and Nutrient Solution on the Anatomical Characteristics of Grapefruit and Shaddock Seedlings and Their Interference”. Grassroots Journal of Natural Resources, 9 no. 1: 126-140. https://doi.org/10.33002/nr2581.6853.090106
Mureef, Ghassan Zaid, Ammar Waleed Taha, Khaled Abdullah Sahar and Ali Ahmed Ali. 2026. “The Effect of Spraying with Jasmonic Growth Regulator and Nutrient Solution on the Anatomical Characteristics of Grapefruit and Shaddock Seedlings and Their Interference”. Grassroots Journal of Natural Resources, 9 (1): 126-140. https://doi.org/10.33002/nr2581.6853.090106
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