Published By:
(ISSN:2581-6853; CODEN: GJNRA9; DOI: 10.33002/nr2581.6853) is an international, scientific double blind peer-reviewed open access journal published 3 times a year online by The Grassroots Institute.
Impact Factor: exaly
Open Access—free for readers, with article processing charges (APC) paid by authors or their institutions.
High Visibility: Indexed in Web of Science (Zoological Record, Biosis Previews, Biological Abstracts, Biosis Full Coverage Unique, and CAB Abstracts), EBSCO and other databases.
Time for Processing: Provisional acceptance of the submitted article is given in 1 week time. After consent of author(s), manuscript is peer-reviewed and a first decision provided to authors in 2-4 weeks after submission.
Recognition of Reviewers: The reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in the journal, in appreciation of the work done. Reviewers also receive Certificate for their voluntary service.
Coordinated and published by The Grassroots Institute, the Grassroots Journal of Natural Resources (GJNR) is an international journal dedicated to the latest advancements in natural resources throughout the world. The goal of this journal is to provide a platform for scientists, social scientists, policy analysts, managers and practitioners (on all academic and professional levels) all over the world to promote, discuss and share various new issues and developments in different arenas of natural resources.
Abderrezak Nimour*1, Fouzia Benourad2
1Plant Protection Laboratory, Department of Agricultural Sciences, Faculty of Natural and Life Sciences, Abdelhamid Ibn Badis University, Mostaganem, Algeria.
Email: abderrezak.nimour.etu@univ-mosta.dz | ORCID: https://orcid.org/0009-0006-0675-8789
2Plant Protection Laboratory, Department of Agricultural Sciences, Faculty of Natural and Life Sciences, Abdelhamid Ibn Badis University, Mostaganem, Algeria.
Email: fouzia.benourad@univ-mosta.dz | ORCID: https://orcid.org/0000-0002-1226-5888
*Corresponding author
Grassroots Journal of Natural Resources, 9(1): 1-22. Doi: https://doi.org/10.33002/nr2581.6853.090101
Received: 26 February 2026
Reviewed: 27 March 2026
Provisionally Accepted: 30 March 2026
Revised: 31 March 2026
Finally Accepted: 11 April 2026
Published: 28 April 2026
Synthetic fungicides are widely used to control Alternaria alternata, but they can cause resistance and environmental problems. Therefore, safer and natural alternatives are needed. This study investigates aqueous extracts from Origanum vulgare (oregano) aerial parts and Punica granatum (pomegranate) peels, prepared using two extraction methods: ultrasonication method (80ºC for 40 min) and pressurized superheated water (121ºC for 7 min). FT-IR analysis confirmed the presence of phenolic compounds, including rosmarinic acid in oregano and ellagitannins in pomegranate. The extraction method influenced the composition of the extracts: superheated water enhanced carbohydrate-related compounds, while ultrasonication improved phenolic compounds. Pomegranate extracts contained higher total phenolics and tannins, whereas oregano extracts were richer in flavonoids. Antioxidant activity was evaluated using DPPH and FRAP assays. Pomegranate extracts showed stronger antioxidant activity, with lower IC50 values. Antifungal tests showed that both extracts inhibited the growth of A. alternata in a dose-dependent manner. The oregano extract obtained by ultrasonication showed the highest inhibition (85.68%) at 300 mg/mL, while the pomegranate extract obtained by superheated water was more effective at a moderate concentration (150 mg/mL). Additionally, both extracts substantially reduced the spore production of A. alternata compared to the control. These results suggest that plant extracts, especially from pomegranate and oregano, could be used as safe and effective natural biofungicides.
Ultrasonic; Superheated water; Origanum vulgare; Punica granatum; Biological activity
Nimour, A. and Benourad, F. (2026). Impact of Plant Matrix and Extraction Techniques on Phenolic Profiling and Biological Activity of Pomegranate (Punica granatum) peel and Oregano (Origanum vulgare) aerial parts Aqueous Extracts. Grassroots Journal of Natural Resources, 9(1): 1-22. Doi: https://doi.org/10.33002/nr2581.6853.090101
Nimour, A., & Benourad, F. (2026). Impact of Plant Matrix and Extraction Techniques on Phenolic Profiling and Biological Activity of Pomegranate (Punica granatum) peel and Oregano (Origanum vulgare) aerial parts Aqueous Extracts. Grassroots Journal of Natural Resources, 9(1), 1-22. https://doi.org/10.33002/nr2581.6853.090101
Nimour A., Benourad F. Impact of Plant Matrix and Extraction Techniques on Phenolic Profiling and Biological Activity of Pomegranate (Punica granatum) peel and Oregano (Origanum vulgare) aerial parts Aqueous Extracts. Grassroots Journal of Natural Resources, 2026, 9 (1), 1-22. https://doi.org/10.33002/nr2581.6853.090101
Nimour, Abderrezak, Benourad, Fouzia. 2026. “Impact of Plant Matrix and Extraction Techniques on Phenolic Profiling and Biological Activity of Pomegranate (Punica granatum) peel and Oregano (Origanum vulgare) aerial parts Aqueous Extracts”. Grassroots Journal of Natural Resources, 9 no. 1: 1-22. https://doi.org/10.33002/nr2581.6853.090101
Nimour, Abderrezak and Fouzia Benourad. 2026. “Impact of Plant Matrix and Extraction Techniques on Phenolic Profiling and Biological Activity of Pomegranate (Punica granatum) peel and Oregano (Origanum vulgare) aerial parts Aqueous Extracts”. Grassroots Journal of Natural Resources, 9 (1): 1-22. https://doi.org/10.33002/nr2581.6853.090101
| Crossref: | https://doi.org/10.33002/nr2581.6853.090101 |
| EuroPub: | |
| Scilit: | |
| Academia.edu: | |
| Dimensions: | |
| ZENODO: | |
| OpenAIRE: | |
| Scribd: | |
| Research Gate: | |
| Google Scholar: |
| Internet Archive: | |
| WorldCat: |
© 2026 by the author(s). Licensee Grassroots Journal of Natural Resources. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). We allow to freely share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, even commercially) with a legal code: https://creativecommons.org/licenses/by/4.0/legalcode.
Grassroots Journal of Natural Resources by The Grassroots Institute is licensed under a Creative Commons Attribution 4.0 International License. Based on a work at www.grassrootsjournals.org.
Go to Top
